Gene Expression Modulation in Rat Skeletal Muscle by Denervation-induced Atrophy / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
; : 84-91, 2008.
Article
em Ko
| WPRIM
| ID: wpr-126979
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
To understand the modulation of genes by atrophy, differential expression of genes in normal and denervated skeletal muscle was investigated by DNA chip technology. MATERIALS ANDMETHODS:
Sciatic nerve and femoral nerve were resected in right leg of rat to make the muscle atrophy model. Muscle tissues from the gastrocnemius of normal and denervated legs were homogenized and RNA were extracted. Dyes were labelled during reverse transcription and hybridization was done into the DNA chip which is consisted of about 5,000 probes.RESULTS:
By statistical analysis, 39 genes were selected as differentially expressed genes by atrophy. 15 known genes up-regulated by atrophy were genes related to immune response, extracellular matrix, andsignal transduction in plasma membrane. 7 known genes down-regulated by atrophy were genes related to cell growth and proliferation, intracellular signal transduction, and energy metabolism. Some unknown gene functions were analysed by bioinformatics analysis and they were highly homologous genes with McKusick-Kaufman syndrome protein, ADP-ribosylation factor-like 4, and component X of pyruvate dehydrogenase complex.CONCLUSION:
These results suggest that reduction of energy metabolism, activation of cholesterol exclusion, and changes on signal transduction pathway are involved in the process of atrophy by denervation in skeletal muscle of rat.Palavras-chave
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Assunto principal:
Oxirredutases
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Atrofia
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Nervo Isquiático
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Doenças Uterinas
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Anormalidades Múltiplas
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RNA
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Atrofia Muscular
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Transdução de Sinais
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Expressão Gênica
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Membrana Celular
Limite:
Animals
Idioma:
Ko
Revista:
Journal of Korean Orthopaedic Research Society
Ano de publicação:
2008
Tipo de documento:
Article