Corn silk induces nitric oxide synthase in murine macrophages
Experimental & Molecular Medicine
; : 545-550, 2004.
Article
em En
| WPRIM
| ID: wpr-13637
Biblioteca responsável:
WPRO
ABSTRACT
Corn silk has been purified as an anticoagulant previously and the active component is a polysaccharide with a molecular mass of 135 kDa. It activates murine macrophages to induce nitric oxide synthase (NOS) and generate substantial amounts of NO in time and dose-dependent manners. It was detectable first at 15 h after stimulation by corn silk, peaked at 24 h, and undetectable by 48 h. Induction of NOS is inhibited by pyrolidine dithiocarbamate (PDTC) and genistein, an inhibitor of nuclear factor kappa B (NF-kappaB) and tyrosine kinase, respectively, indicating that iNOS stimulated by corn silk is associated with tyrosine kinase and NF-kappaB signaling pathways. IkappaB-alpha degradation was detectible at 10 min, and the level was restored at 120 min after treatment of corn silk. Corn silk induced nuclear translocation of NF-kappaB by phosphorylation and degradation of IkappaB-alpha.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosforilação
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Polissacarídeos
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Pirrolidinas
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Tiocarbamatos
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Proteínas Tirosina Quinases
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Extratos Vegetais
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NF-kappa B
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Óxido Nítrico Sintase
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Zea mays
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Genisteína
Limite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2004
Tipo de documento:
Article