Molecular Epidemiologic Analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction-Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE) / 감염과화학요법
Infection and Chemotherapy
; : 5-10, 2012.
Article
em Ko
| WPRIM
| ID: wpr-141454
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli, and also assessed the use of PFGE as an alternative method. MATERIALS AND METHODS: IRS-PCR assay was performed using combinations of adaptors for XbaI and HhaI restriction sites on clinical isolates of E. coli (n=51). We compared the discriminatory power, quality and efficiency of IRS-PCR to PFGE. RESULTS: In E. coli, PFGE discriminated 39 (76.4%) and IRS-PCR discerned 41 (80.3%) of the total 51 strains. It took much less time to complete IRS-PCR (one day) than PFGE (at least 4 days). CONCLUSIONS: IRS-PCR is a more sensitive and rapid alternative to PFGE for molecular epidemiologic analysis of E. coli.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Beta-Lactamases
/
Reação em Cadeia da Polimerase
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Eletroforese em Gel de Campo Pulsado
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Escherichia
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Escherichia coli
Idioma:
Ko
Revista:
Infection and Chemotherapy
Ano de publicação:
2012
Tipo de documento:
Article