The Prostaglandin E Receptor Agonists Increase Granulocyte Macrophage Colony-Stimulating Factor in Prostate Cancer Cells / 대한비뇨기과학회지
Korean Journal of Urology
; : 1272-1278, 2004.
Article
em Ko
| WPRIM
| ID: wpr-144324
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The predilection for prostate carcinoma cells to metastasize to bone suggests the hypothesis that bone and/or bone marrow-derived factors may promote prostate carcinoma cell growth and/or their survival. To date, little work has been performed to characterize the nature of granulocyte macrophage colony-stimulating factor (GM-CSF) and the expression of prostaglandin E2 receptors (EPs) in prostate cancer (PC) cells. The aim of this study is to evaluate the effects of GM-CSF on cell proliferation and the effects of EP agonists on the production of GM-CSF in the PC-3 cells. MATERIALS AND METHODS: The bone-derived PC-3 cell line was used in this study. Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the mRNA expression of EP1, 2, 3 and 4 and hGM- CSF. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was done to estimate the viability of PC-3 cells after hGM-CSF treatment. hGM-CSF was measured by enzyme-linked immunosorbent assay (ELISA) after treatments with the EPs agonist at 10(-10), 10(-8), 10(-6)M, respectively. RESULTS: EP2, 3 and 4 and hGM-CSF were expressed in the PC-3 cell line. Viability of the PC-3 cells was significantly increased by hGM-CSF administration in a dose- and time-dependent manner. Also, our data indicated that EP2, 3 and especially 4 agonists induced a significant dose- dependent increase in hGM-CSF production in comparison to the control group in the conditioned ELISA medium. CONCLUSIONS: These results suggest that GM-CSF may be part of a network of an autocrine-regulatory loop system that modulates the biologic activity of prostate carcinoma cells. Our data suggest that GM-CSF and EPs may represent a possible novel therapeutic target that manipulates the proliferative rate of prostate tumors.
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Índice:
WPRIM
Assunto principal:
Próstata
/
Neoplasias da Próstata
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RNA Mensageiro
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Ensaio de Imunoadsorção Enzimática
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Dinoprostona
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Linhagem Celular
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Reação em Cadeia da Polimerase
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Fator Estimulador de Colônias de Granulócitos e Macrófagos
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Fator Estimulador de Colônias de Macrófagos
/
Receptores de Prostaglandina E
Idioma:
Ko
Revista:
Korean Journal of Urology
Ano de publicação:
2004
Tipo de documento:
Article