IL-1beta Acts in Synergy with Endogenous IL-1beta in A375-S2 Human Melanoma Cell Apoptosis Through Mitochondrial Pathway
Journal of Korean Medical Science
; : 555-561, 2005.
Article
em En
| WPRIM
| ID: wpr-147627
Biblioteca responsável:
WPRO
ABSTRACT
Interleukin-1beta (IL-1beta) is a pivotal proinflammatory cytokine. To investigate the mechanism of IL-1beta-induced cell death in human malignant melanoma A375-S2 cells, MTT assay, photomicroscopical observation, DNA agarose gel electrophoresis, radioimmunoassay and Western blot analysis were carried out. IL-1beta did not only induce nuclear condensation and DNA fragmentation, but also increased degradation of two substrates of caspase-3, poly ADP-ribose polymerase (PARP) and inhibitor of caspase-activated DNase (ICAD). Simultaneously, release of precursor of IL-1beta (pro-IL-1beta) and endogenous IL-1beta production were involved in the apoptotic process. IL-1beta enhanced the ratio of Bax/Bcl-2 and Bax/Bcl-xL expression and up-regulated apoptosis inducing factor (AIF) expression, which required the activation of downstream caspases. These results suggest that IL-1beta induces endogenous IL-1beta production, enhances cleavage of caspase downstream substrates and promotes mitochondria mediated apoptosis in A375-S2 cells.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fatores de Tempo
/
Estudo Comparativo
/
Sobrevivência Celular
/
Western Blotting
/
Interleucina-6
/
Interleucina-1
/
Linfotoxina-alfa
/
Poli(ADP-Ribose) Polimerases
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
Limite:
Humans
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2005
Tipo de documento:
Article