Triptolide inhibits ovarian cancer cell invasion by repression of matrix metalloproteinase 7 and 19 and upregulation of E-cadherin
Experimental & Molecular Medicine
; : 633-641, 2012.
Article
em En
| WPRIM
| ID: wpr-149765
Biblioteca responsável:
WPRO
ABSTRACT
Triptolide, a compound extracted from the traditional Chinese medicine preparation of Tripterygium wilfordii Hook F., has been reported to have anti-inflammatory and anti-cancer activities. However, its effect on ovarian cancer invasion is unknown. We observed that MMP7 and MMP19 expression increased in ovarian cancer tissue. Triptolide treatment inhibited the migration and invasion of ovarian cancer cells SKOV3 and A2780 at the concentration of 15 nM. We also observed that triptolide suppressed MMP7 and MMP19 promoter activity in a dose-dependent manner, down-regulating the expressions of these promoters on mRNA and protein level. Moreover, triptolide enhanced E-cadherin expression in ovarian cancer cells. In vivo, triptolide inhibited tumor formation and metastasis in nude mice, and suppressed MMP7 and MMP19 expression; it also enhanced E-cadherin expression in tumor in a dose-dependent manner. Over expression of MMP7 and MMP19, or suppression of E-cadherin expression partially abolished the inhibitory effect of triptolide on invasion of ovarian cancer cells. To summarize, triptolide significantly inhibited the migration and invasion of ovarian cancer cells by suppression of MMP7 and MMP19 and up-regulation of E-cadherin expression. This study shows that triptolide is a good candidate for the treatment of ovarian cancer and reduction of metastasis.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Neoplasias Ovarianas
/
Fenantrenos
/
Caderinas
/
Regulação Enzimológica da Expressão Gênica
/
Regulação para Cima
/
Movimento Celular
/
Regiões Promotoras Genéticas
/
Paclitaxel
/
Cistadenocarcinoma Seroso
/
Antineoplásicos Alquilantes
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2012
Tipo de documento:
Article