The pH-dependent effects of combining ethanol with fluoride on proton permeability in Streptococcus mutans / 대한구강보건학회지
Journal of Korean Academy of Oral Health
; : 255-260, 2016.
Article
em En
| WPRIM
| ID: wpr-156067
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVES: The aim of this research was to determine the pH-dependent changes in F-ATPase activity and proton fluxes in Streptococcus mutans (S. mutans) as induced by varying the concentration of fluoride ±10 mM (0.058% (v/v)) ethanol. METHODS: S. mutans UA159 was grown in Brain Heart Infusion medium at pH 4.8, 6.8, or 8.8. The F-ATPase assay was initiated by the addition of ATP, and stopped by adding 10% trichloroacetic acid. For the proton flux assay, bacterial suspensions were titrated to pH 4.6 with 0.5 M HCl, and then 0.5 M HCl was added to decrease the pH values in units of approximately 0.4 pH. The subsequent increase in pH was monitored using a glass electrode. To disrupt the cell membrane, 10% (v/v) butanol was added to the suspensions after 80 minutes. RESULTS: At all pH levels, fluoride ±10 mM ethanol not only decreased F-ATPase activity but also increased the proton permeability of S. mutans. The largest effects were observed at pH 4.8. Ethanol enhanced these effects only at pH 4.8. CONCLUSIONS: A very low concentration of ethanol enhanced the action of fluoride on F-ATPase activity and the proton permeability in S. mutans at acidic pH levels. We expect that low concentrations of ethanol may be used together with fluoride and/or other anticaries agents to develop more effective anticaries preparations.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Permeabilidade
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Prótons
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Streptococcus
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Streptococcus mutans
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Suspensões
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Ácido Tricloroacético
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Encéfalo
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Membrana Celular
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Trifosfato de Adenosina
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Etanol
Idioma:
En
Revista:
Journal of Korean Academy of Oral Health
Ano de publicação:
2016
Tipo de documento:
Article