Expression Profiles of F4/80 and Nestin in Ocular Immune Cells Following Pharmaceutically Induced Retinal Degeneration in Adult Mice
Journal of the Korean Ophthalmological Society
; : 945-953, 2013.
Article
em Ko
| WPRIM
| ID: wpr-160293
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To evaluate the expression patterns of F4/80 and nestin in the ciliary body and the optic nerve following N-methyl-N-nitrosourea (NMU)-induced retinal degeneration in adult mice. METHODS: After intraperitoneal injection of MNU (60 mg/kg) in adult mice, the eyes were enucleated at 2, 4, 7 and 30 days. Hematoxylin and eosin (H&E) stain, terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stain and immunohistochemical stains of F/80 and nestin were performed. RESULTS: After MNU treatment, the photoreceptors were destroyed by cell apoptosis. According to immunohistochemistry, F4/80 and nestin were not co-expressed in the control group, but F4/80 was expressed within the ciliary body and optic nerve in the MNU-treated group; the expression of nestin also increased. In the outer nuclear layer, F4/80 and nestin co-expressing cells were observed. CONCLUSIONS: In response to retinal damage, the F4/80 and nestin co-expressing cells migrated to the retina from the ciliary body and optic nerve and were activated.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Nervo Óptico
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Retina
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Degeneração Retiniana
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Retinaldeído
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Imuno-Histoquímica
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Corpo Ciliar
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Apoptose
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Amarelo de Eosina-(YS)
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Corantes
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Olho
Limite:
Animals
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Humans
Idioma:
Ko
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2013
Tipo de documento:
Article