Role of Staphylococcal Superantigen in Atopic Dermatitis: Influence on Keratinocytes
Journal of Korean Medical Science
; : 315-323, 2006.
Article
em En
| WPRIM
| ID: wpr-162122
Biblioteca responsável:
WPRO
ABSTRACT
Staphylococcus aureus may perform an crucial function in atopic dermatitis (AD), via the secretion of superantigens, including staphylococcal enterotoxins (SE) A or B, and toxic shock syndrome toxin-1 (TSST-1). Dysregulated cytokine production by keratinocytes (KCs) upon exposure to staphylococcal superantigens (SsAgs) may be principally involved in the pathophysiology of AD. We hypothesized that lesional KCs from AD may react differently to SsAgs compared to nonlesional skin or normal skin from nonatopics. We conducted a comparison of HLA-DR or CD1a expression in lesional skin as opposed to that in nonlesional or normal skin by immunohistochemistry (IHC). We also compared, using ELISA, the levels of IL-1alpha, IL-1beta, and TNF-alpha secreted by cultured KCs from lesional, nonlesional, and normal skin, after the addition of SEA, SEB and TSST-1. IHC revealed that both HLA-DR and CD1a expression increased significantly in the epidermis of lesional skin versus nonlesional or normal skin in quite a similar manner. IL-1alpha, IL-1beta, and TNF-alpha secretion was also significantly elevated in the cultured KCs from lesional skin after the addition of SsAgs. Our results indicated that KCs from lesional skin appear to react differently to SsAgs and increased proinflammatory cytokine production in response to SsAgs may contribute to the pathogenesis of AD.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Staphylococcus aureus
/
Toxinas Bacterianas
/
Sequência de Bases
/
Antígenos HLA-DR
/
Estudos de Casos e Controles
/
Queratinócitos
/
Interleucina-1
/
Fator de Necrose Tumoral alfa
/
Superantígenos
/
DNA Complementar
Tipo de estudo:
Observational_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2006
Tipo de documento:
Article