Isolation, Characterization and Growth Kinetic Comparison of Bone Marrow and Adipose Tissue Mesenchymal Stem Cells of Guinea Pig
International Journal of Stem Cells
; : 115-123, 2016.
Article
em En
| WPRIM
| ID: wpr-171023
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Mesenchymal stem cells (MSCs) from different sources have different characteristics. Moreover, MSCs are not isolated and characterized in Guinea pig for animal model of cell therapy. AIM OF THE WORK: was the isolating of bone marrow MSCs (BM-MSCs) and adipose tissue MSCs (AT-MSCs) from Guinea pig and assessing their characteristics. MATERIAL AND METHODS: In this study, bone marrow and adipose tissue were collected from three Guinea pigs and cultured and expanded through eight passages. BM-MSCs and AT-MSCs at passages 2, 5 and 8 were seeded in 24-well plates in triplicate. Cells were counted from each well 1~7 days after seeding to determine population doubling time (PDT) and cell growth curves. Cells of passage 3 were cultured in osteogenic and adipogenic differentiation media. RESULTS: BM-MSCs and AT-MSCs attached to the culture flask and displayed spindle-shaped morphology. Proliferation rate of AT-MSCs in the analyzed passages was more than BM-MSCs. The increase in the PDT of MSCs occurs with the increase in the number of passages. Moreover, after culture of BM-MSCs and AT-MSCs in differentiation media, the cells differentiated toward osteoblasts and adipocytes as verified by Alizarin Red staining and Oil Red O staining, respectively. CONCLUSION: BM-MSCs and AT-MSCs of Guinea pig could be valuable source of multipotent stem cells for use in experimental and preclinical studies in animal models.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Osteoblastos
/
Medula Óssea
/
Tecido Adiposo
/
Guiné
/
Adipócitos
/
Modelos Animais
/
Células-Tronco Multipotentes
/
Células-Tronco Mesenquimais
/
Terapia Baseada em Transplante de Células e Tecidos
/
Cobaias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Africa
Idioma:
En
Revista:
International Journal of Stem Cells
Ano de publicação:
2016
Tipo de documento:
Article