Chitin from Cuttlebone Activates Inflammatory Cells to Enhance the Cell Migration
Biomolecules & Therapeutics
; : 333-338, 2015.
Article
em En
| WPRIM
| ID: wpr-180158
Biblioteca responsável:
WPRO
ABSTRACT
Our previous report showed that the extract from cuttlebone (CB) had wound healing effect in burned lesion of rat and the extract was identified as chitin by HPLS analysis. We herein investigated the morphology in CB extract using scanning electron microscope (SEM). Chitin was used as a control. There is no difference in morphology between CB extract and chitin. We also assessed the role of CB extract on the production of inflammatory mediators using murine macrophages and the migration of inflammatory cells. The extract induced the production of nitric oxide (NO) in macrophages. While the extract of CB itself stimulated macrophages to increase the expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6, CB extract suppressed the production of those cytokines by LPS. CB extract also induced the production of mouse IL-8 which is related to the cell migration, and treatment with CB enhanced fibroblast migration and invasion. Therefore, our results suggest that CB activates inflammatory cells to enhance the cell migration.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Cicatrização
/
Queimaduras
/
Movimento Celular
/
Quitina
/
Citocinas
/
Interleucina-8
/
Interleucinas
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Interleucina-6
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Fator de Necrose Tumoral alfa
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Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomolecules & Therapeutics
Ano de publicação:
2015
Tipo de documento:
Article