Ceramides and Cell Signaling Molecules in Psoriatic Epidermis: Reduced Levels of Ceramides, PKC-alpha, and JNK
Journal of Korean Medical Science
; : 95-99, 2006.
Article
em En
| WPRIM
| ID: wpr-181110
Biblioteca responsável:
WPRO
ABSTRACT
Ceramides are the main lipids in the stratum corneum and are generated during cellular stress and apoptosis by de novo synthesis or by the action of sphingomyelinase. In addition, they are lipid second messengers produced by sphingolipid metabolism and trigger important cell responses, including protein kinase C-alpha (PKC-alpha) activation and the stimulation of signal transduction pathways with apoptosis and stress-activated protein kinases (SAPK), such as c-jun N-terminal kinase (JNK). Thus, ceramides have anti-proliferative and apoptotic effects. This study measured the changes in the levels of epidermal ceramides and ceramide-related apoptotic signaling molecules in psoriasis patients. Samples from lesional and non-lesional epidermis were obtained from psoriasis patients. Total ceramides were fractionated using thin-layer chromatography, and the levels of PKC-alpha and JNK expression were measured using Western blot analysis with specific antibodies. The ceramide level was reduced significantly, and this was associated with the downregulation of apoptotic signaling molecules, such as PKC-alpha and JNK, in the lesional epidermis of psoriasis patients. These results suggest that the decreased level of ceramides downregulates the apoptotic pathway, leading to epidermal proliferation in psoriasis.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Psoríase
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Índice de Gravidade de Doença
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Transdução de Sinais
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Ceramidas
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Western Blotting
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Cromatografia em Camada Fina
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Apoptose
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Proteínas Quinases JNK Ativadas por Mitógeno
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Epiderme
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Proteína Quinase C-alfa
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Journal of Korean Medical Science
Ano de publicação:
2006
Tipo de documento:
Article