Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides
Journal of Veterinary Science
; : 399-407, 2014.
Article
em En
| WPRIM
| ID: wpr-194858
Biblioteca responsável:
WPRO
ABSTRACT
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4+ T cells and IFN-gamma-producing CD8+ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3+, CD3+CD4+CD8-, and CD3+CD4-CD8+ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oligodesoxirribonucleotídeos
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Imunoglobulina A
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Imunoglobulina G
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Administração Intranasal
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Vacinas Virais
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Vacinas Sintéticas
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Adenoviridae
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Circovirus
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Infecções por Circoviridae
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Imunidade nas Mucosas
Limite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Ano de publicação:
2014
Tipo de documento:
Article