Influence of Bacterial Presence on Biofilm Formation of Candida albicans
Yonsei Medical Journal
; : 449-458, 2014.
Article
em En
| WPRIM
| ID: wpr-19541
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Candida albicans is an opportunistic pathogen that is commonly found in human microflora. Biofilm formation (BF) is known as a major virulence factor of C. albicans. The aim of this study was to examine the influence of bacterial presence on biofilm formation of C. albicans. MATERIALS ANDMETHODS:
The BF of Candida was investigated when it was co-cultured with C. albicans (C. albicans 53, a yeast with a low BF ability, and C. albicans 163, a yeast with high BF ability) and bacteria. BF was assessed with XTT reduction assay. A scanning electron microscope was used to determine the structure of the biofilm, and real-time reverse transcriptase polymerase chain reaction was used to amplify and quantify hyphae-associated genes.RESULTS:
Co-culturing with two different types of bacteria increased the BF value. Co-culturing with C. albicans 53 and 163 also increased the BF value compared to the value that was obtained when the C. albicans was cultured individually. However, co-culturing with bacteria decreased the BF value of C. albicans, and the BF of C. albicans 163 was markedly inhibited. The expression of adherence and morphology transition related genes were significantly inhibited by co-culturing with live bacteria.CONCLUSION:
Bacteria have a negative effect on the formation of biofilm by C. albicans. This mechanism is the result of the suppression of genes associated with the hyphae transition of C. albicans, and bacteria particles physically affected the biofilm architecture and biofilm formation.Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Arquitetura
/
Bactérias
/
Virulência
/
Leveduras
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Candida
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Candida albicans
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Biofilmes
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Técnicas de Cocultura
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Hifas
Limite:
Humans
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2014
Tipo de documento:
Article