Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant / 대한수의학회지
Korean Journal of Veterinary Research
; : 211-216, 2013.
Article
em Ko
| WPRIM
| ID: wpr-200777
Biblioteca responsável:
WPRO
ABSTRACT
This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosfotransferases
/
Salmonella
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Salmonella enteritidis
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Baço
/
Ceco
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Galinhas
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Colo
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Proteção Cruzada
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Fígado
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Formação de Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2013
Tipo de documento:
Article