Atractylochromene Is a Repressor of Wnt/beta-Catenin Signaling in Colon Cancer Cells
Biomolecules & Therapeutics
; : 26-30, 2015.
Article
em En
| WPRIM
| ID: wpr-20364
Biblioteca responsável:
WPRO
ABSTRACT
Wnt/beta-catenin signaling pathway was mutated in about 90% of the sporadic and hereditary colorectal cancers. The abnormally activated beta-catenin increases the cancer cell proliferation, differentiation and metastasis through increasing the expression of its oncogenic target genes. In this study, we identified an inhibitor of beta-catenin dependent Wnt pathway from rhizomes of Atractylodes macrocephala Koidzumi (Compositae). The active compound was purified by activity-guided purification and the structure was identified as 2,8-dimethyl-6-hydroxy-2-(4-methyl-3-pentenyl)-2H-chromene (atractylochromene, AC). AC suppressed beta-catenin/T-cell factor transcriptional activity of HEK-293 reporter cells when they were stimulated by Wnt3a or inhibitor of glycogen synthase kinase-3beta. AC down-regulated the nuclear level of beta-catenin through the suppression of galectin-3 mediated nuclear translocation of beta-catenin in SW-480 colon cancer cells. Furthermore, AC inhibits proliferation of colon cancer cell. Taken together, AC from A. macrocephala might be a potential chemotherapeutic agent for the prevention and treatment of human colon cancer.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Neoplasias Colorretais
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Glicogênio Sintase
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Neoplasias do Colo
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Rizoma
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Atractylodes
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Galectina 3
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Proliferação de Células
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Beta Catenina
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Via de Sinalização Wnt
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Metástase Neoplásica
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biomolecules & Therapeutics
Ano de publicação:
2015
Tipo de documento:
Article