Osteoprotegerin is present on the membrane of osteoclasts isolated from mouse long bones
Experimental & Molecular Medicine
; : 347-352, 2002.
Article
em En
| WPRIM
| ID: wpr-203702
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WPRO
ABSTRACT
Osteoprotegerin (OPG), a member of the tumor necrosis factor receptor superfamily, is known to inhibit osteoclastogenesis by acting as a soluble decoy receptor for the receptor activator of NF-kB ligand (RANKL). We report the presence of OPG on the membrane of osteoclasts and the possibility of the direct action of OPG on them. Highly pure osteoclast precursors were isolated from mouse long bones and induced to differentiate into mature osteoclasts by M-CSF and soluble RANKL (sRANKL). The presence of OPG on the membrane of these cells was confirmed by western blotting and immunostaining. Furthermore, sRANKL was found to be bound to the OPG on the osteoclast precursors. These results suggest that OPG might have a new role during the differentiation of osteoclasts beyond its role as a soluble decoy receptor. The mechanism of the existence of OPG on osteoclast precursors remains to be found.
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Índice:
WPRIM
Assunto principal:
Osteoclastos
/
Células-Tronco
/
Osso e Ossos
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Glicoproteínas de Membrana
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Glicoproteínas
/
Proteínas de Transporte
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Diferenciação Celular
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Membrana Celular
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Células Cultivadas
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Fator Estimulador de Colônias de Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2002
Tipo de documento:
Article