A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis
The Korean Journal of Parasitology
; : 251-256, 2014.
Article
em En
| WPRIM
| ID: wpr-207961
Biblioteca responsável:
WPRO
ABSTRACT
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4+ and CD8+ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Baço
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Administração Intranasal
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Portadores de Fármacos
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Vacina BCG
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Vacinas Sintéticas
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Linfócitos T CD4-Positivos
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Galinhas
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Adjuvantes Imunológicos
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Vacinas Protozoárias
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Interleucina-2
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2014
Tipo de documento:
Article