Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions
The Korean Journal of Parasitology
;
: 373-378, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-220300
ABSTRACT
To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Trichomonas vaginalis
/
Organelas
/
Regulação Enzimológica da Expressão Gênica
/
Regulação da Expressão Gênica
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Meios de Cultura
/
Piruvato Sintase
/
Ferredoxinas
/
Hidrogênio
/
Hidrogenase
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2006
Tipo de documento:
Artigo
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