Effect of Pneumoperitoneum on Oxidative Stress and Inflammation via the Arginase Pathway in Rats
Yonsei Medical Journal
; : 238-246, 2016.
Article
em En
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| ID: wpr-220776
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ABSTRACT
PURPOSE:
Oxidative stress during CO2 pneumoperitoneum is reported to be associated with decreased bioactivity of nitric oxide (NO). However, the changes in endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), and arginase during CO2 pneumoperitoneum have not been elucidated. MATERIALS ANDMETHODS:
Thirty male Sprague-Dawley rats were randomized into three groups. After anesthesia induction, the abdominal cavities of the rats of groups intra-abdominal pressure (IAP)-10 and IAP-20 were insufflated with CO2 at pressures of 10 mm Hg and 20 mm Hg, respectively, for 2 hours. The rats of group IAP-0 were not insufflated. After deflation, plasma NO was measured, while protein expression levels and activity of eNOS, iNOS, arginase (Arg) I, and Arg II were analyzed with aorta and lung tissue samples.RESULTS:
Plasma nitrite concentration and eNOS expression were significantly suppressed in groups IAP-10 and IAP-20 compared to IAP-0. While expression of iNOS and Arg I were comparable between the three groups, Arg II expression was significantly greater in group IAP-20 than in group IAP-0. Activity of eNOS was significantly lower in groups IAP-10 and IAP-20 than in group IAP-0, while iNOS activity was significantly greater in group IAP-20 than in groups IAP-0 and IAP-10. Arginase activity was significantly greater in group IAP-20 than in groups IAP-0 and IAP-10.CONCLUSION:
The activity of eNOS decreases during CO2 pneumoperitoneum, while iNOS activity is significantly increased, a change that contributes to increased oxidative stress and inflammation. Moreover, arginase expression and activity is increased during CO2 pneumoperitoneum, which seems to act inversely to the NO system.Palavras-chave
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Índice:
WPRIM
Assunto principal:
Aorta
/
Arginase
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Pneumoperitônio
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Ratos Sprague-Dawley
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Estresse Oxidativo
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Inibidores Enzimáticos
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Óxido Nítrico Sintase Tipo II
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Óxido Nítrico Sintase Tipo III
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Lesão Pulmonar
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Inflamação
Limite:
Animals
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2016
Tipo de documento:
Article