Influence of Interferon alpha-2b on proliferation inhibition and apoptosis induction in HL-60 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 56-58, 2007.
Article
em Zh
| WPRIM
| ID: wpr-230334
Biblioteca responsável:
WPRO
ABSTRACT
To investigate the effects of interferon alpha-2b on proliferation and apoptosis in HL-60 cells, HL-60 cells were cultured in different concentrations of IFN alpha-2b. The morphologic changes were observed by Wright's and acridine orange (AO) and ethidium bromide (EB) staining respectively. Inhibition of proliferation was detected by MTT. Expression of CD13(+) was checked by indirect fluoroimmunoassay. The results showed that apoptosis rate of HL-60 cells assayed by the above-mentioned two methods was (51 +/- 2)% and (78 +/- 3)% respectively and OD(570) values of proliferation inhibited were 1.8 +/- 0.1 and 1.0 +/- 0.1 respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. Morphology and count of CD13(+) cells were changed. CD13(+) cell expression rate was (62 +/- 2)% and (30 +/- 3)% respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 48 hours. It is concluded that IFN(alpha-2b) can enhance the apoptosis of HL-60 cells, inhibit their proliferation, promote their maturation and differentiation.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Proteínas Recombinantes
/
Interferon-alfa
/
Apoptose
/
Antígenos CD13
/
Células HL-60
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Proliferação de Células
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Genética
/
Antineoplásicos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Article