Expression of human interleukin-11 in cell culture and larvae of silkworm / 生物工程学报
Chinese Journal of Biotechnology
; (12): 30-34, 2002.
Article
em Zh
| WPRIM
| ID: wpr-231379
Biblioteca responsável:
WPRO
ABSTRACT
A recombinant transfer vector, pBacIL-11, containing hIL-11 cDNA of 546 nucleotides lacking leader sequence was constructed and co-transfected into BmN cells with linearized BmBacPAK(modified BmNPV) DNA for construction of a recombinant baculovirus carrying the hIL-11 gene. Southern hybridization analysis suggested that the recombinant baculovirus DNA contained hIL-11 cDNA fragment. RNA dot blotting demonstrated that the hIL-11 gene was transcribed. The recombinant baculovirus has a strong infectivity to BmN cell line and to silkworm larvae and pupae. Specific hIL-11 bands were detected from all the samples of cell extract, culture supernatant, haemolymph of larvae and pupae by SDS-PAGE analysis. Biological activity of the expressed product was determined with IL-11 dependent B9-11 cell line and by MTT colorimetric assay, which indicated that biologically active rhIL-11 protein was overexpressed in BmN cell line and in silkworm larvae and pupae.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Pupa
/
Bombyx
/
Expressão Gênica
/
Baculoviridae
/
Interleucina-11
/
Técnicas de Cultura de Células
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Biologia Celular
/
Vetores Genéticos
/
Genética
/
Larva
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Article