Sequence analysis on measles viruses isolated in Shanghai in 2005 / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 165-168, 2007.
Article
em Zh
| WPRIM
| ID: wpr-232328
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To ascertain the genetic characterization and genotype of measles viruses isolated in Shanghai region, in 2005.</p><p><b>METHODS</b>Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus of nucleprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain published in GenBank.</p><p><b>RESULTS</b>4 measles viruses were isolated from 10 throat swab specimens, and the sequence analysis indicated that they belonged to H1 genotype. The homogeneity of 450 nucleotides in the C terminal of the N gene was at 98%-98.2% as compared to H1 genotype (China93-7). They differed from genotype H2 (China94-1) at 6.4%-6.9% and from genotype A (Edmonston) at 6.7%-6.9%, from measles vaccine (Shanghail91) at 7.6%-8.0%. They differed from the other measles viral strain isolated in China in 1993 - 2005 at 0.2%-3.7%. The variation within 4 isolated measles viruses was at 0.7%-1.3%.</p><p><b>CONCLUSION</b>It was H1 genotype measles viruses,which are the native viruses in China that led to the outbreak of measles in Shanghai, in 2005.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
China
/
Epidemiologia
/
Surtos de Doenças
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Análise de Sequência de DNA
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Genética
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Genótipo
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Sarampo
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Vírus do Sarampo
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2007
Tipo de documento:
Article