Differential expressions of microRNAs and their predicted targets in liver transplant recipients with long-term stable survival / 南方医科大学学报
Journal of Southern Medical University
; (12): 1557-1563, 2015.
Article
em Zh
| WPRIM
| ID: wpr-232571
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) differs between liver transplant recipients with long-term stable survival and those with acute rejection.</p><p><b>METHODS</b>Twenty-nine liver transplant recipients with long-term stable survival (STA) group, 10 recipients with acute rejection (RJ group), and 17 healthy subjects (control group) were recruited for genome-wide microarray analysis of miRNA expressions in the PBMCs. The differentially expressed miRNAs among the 3 groups were validated by real-time PCR, and the targets of these miRNAs were predicted.</p><p><b>RESULTS</b>Compared with the RJ group, the STA group showed down-regulation of 13 miRNAs in the PBMCs. Of these down-regulated miRNAs, miRNA-18b, miRNA-340 and miRNA-106b were validated by real-time PCR, and the latter two miRNAs were predicted to target the TGF-β pathway.</p><p><b>CONCLUSIONS</b>The differentially expressed miRNAs in liver transplant recipients with long-term stable survival, namely miRNA-18b, miRNA-340 and miRNA-106b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Leucócitos Mononucleares
/
Biomarcadores
/
Estudos de Casos e Controles
/
Regulação para Baixo
/
Taxa de Sobrevida
/
Fator de Crescimento Transformador beta
/
Transplante de Fígado
/
Análise de Sequência com Séries de Oligonucleotídeos
/
MicroRNAs
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Article