Determination of cocaine and benzoylecgonine in guinea pig's hair after a single dose administration by LC-MS/MS / 药学学报
Acta Pharmaceutica Sinica
; (12): 1217-1223, 2008.
Article
em En
| WPRIM
| ID: wpr-232614
Biblioteca responsável:
WPRO
ABSTRACT
A sensitive LC-MS/MS method to determine cocaine and its major metabolite benzoylecgonine in guinea pig' s hair has been established. About 20 mg of decontaminated hair sample was hydrolyzed with 0. 1 mol x L(-1) HCl at 50 degrees C overnight, in the presence of cocaine-d3 and benzoylecgonine-d8 used as internal standards, and then extracted with dichlormethane. The analysis was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Positive electrospray ionization (ESI +) and multiple reactions monitoring (MRM) mode were used. The limit of detection (LOD) for cocaine and benzoylecgonine was 1 pg x mg(-1). The calibration curves of extracted standards were linear over the range from 5 pg x mg(-1) to 250 pg x mg(-1) (r2 > or = 0.9997). The method was validated and applied to the analysis of guinea pig's hair after a single dose administration of cocaine hydrochloride. Cocaine and benzoylecgonine were not only detected, but also quantified in guinea pigs hair.
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Índice:
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Assunto principal:
Química
/
Cromatografia Líquida de Alta Pressão
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Cocaína
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Espectrometria de Massas por Ionização por Electrospray
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Espectrometria de Massas em Tandem
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Cobaias
/
Cabelo
/
Metabolismo
Limite:
Animals
Idioma:
En
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2008
Tipo de documento:
Article