Antiproliferative effect of silencing LSD1 gene on Jurkat cell line and its mechanism / 中华血液学杂志
Chinese Journal of Hematology
; (12): 56-60, 2016.
Article
em Zh
| WPRIM
| ID: wpr-234033
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of silencing LSD1 gene by RNA interference on the proliferation, apoptosis on human lymphocytic leukemia Jurkat cell line and its mechanism.</p><p><b>METHODS</b>The hairpin- like oligonucleotide sequences targeting LSD1 gene was transfected into Jurkat cells by lipofectamine(TM) 2000. The LSD1 mRNA and protein were detected by RQ- PCR and Western blot. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expression of Bcl-2, Bax, procaspase- 3, and histone H3K4me, H3K4me2, H3K4me3, Act- H3, H3K9me were detected by Western blot.</p><p><b>RESULTS</b>LSD1 mRNA was markedly suppressed by the shRNA targeting LSD1. LSD1 shRNA suppressed the proliferation and induced cells apoptosis of Jurkat cells. The cell apoptotic rate was (41.34±3.58)%, (3.45±1.54)%, (1.76±0.52)% in LSD1 shRNA, Neg-shRNA and Blank respectively, the difference among them was statistically significant (P<0.05). LSD1 shRNA down- regulated the expressions of Bcl- 2 and procaspase- 3, and up- regulated the expression of Bax. The methylation of H3K4me1, me2 and acetylation of Act- H3 improved without change of the methylation of H3K4me3.</p><p><b>CONCLUSIONS</b>Deplete of LSD1 gene maybe through modifying the methylation of histone H3K4 to promote the cell apoptosis and inhibit cell growth in Jurkat cell line.</p>
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Assunto principal:
Acetilação
/
RNA Mensageiro
/
Histonas
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Transfecção
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Regulação para Baixo
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Ciclo Celular
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Apoptose
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Células Jurkat
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Proteínas Proto-Oncogênicas c-bcl-2
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RNA Interferente Pequeno
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2016
Tipo de documento:
Article