ZnO, TiO(2), SiO(2,) and Al(2)O(3) nanoparticles-induced toxic effects on human fetal lung fibroblasts / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 661-669, 2011.
Article
em En
| WPRIM
| ID: wpr-235584
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to investigate and compare the toxic effects of four types of metal oxide (ZnO, TiO(2), SiO(2,) and Al(2)O(3)) nanoparticles with similar primary size (∼20 nm) on human fetal lung fibroblasts (HFL1) in vitro.</p><p><b>METHODS</b>The HFL1 cells were exposed to the nanoparticles, and toxic effects were analyzed by using MTT assay, cellular morphology observation and Hoechst 33 258 staining.</p><p><b>RESULTS</b>The results show that the four types of metal oxide nanoparticles lead to cellular mitochondrial dysfunction, morphological modifications and apoptosis at the concentration range of 0.25-1.50 mg/mL and the toxic effects are obviously displayed in dose-dependent manner. ZnO is the most toxic nanomaterials followed by TiO(2), SiO(2), and Al(2)O(3) nanoparticles in a descending order.</p><p><b>CONCLUSION</b>The results highlight the differential cytotoxicity associated with exposure to ZnO, TiO(2), SiO(2), and Al(2)O(3) nanoparticles, and suggest an extreme attention to safety utilization of these nanomaterials.</p>
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Assunto principal:
Patologia
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Propriedades de Superfície
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Titânio
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Óxido de Zinco
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Linhagem Celular
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Sobrevivência Celular
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Química
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Embriologia
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Microscopia de Contraste de Fase
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Apoptose
Limite:
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2011
Tipo de documento:
Article