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Application of solid-phase extraction column for determination of matrine and oxymatrine in Sophora flavescens / 中国中药杂志
Article em Zh | WPRIM | ID: wpr-238635
Biblioteca responsável: WPRO
ABSTRACT
A Cleanert Alumina-N-SPE column (0.5 g/6 mL) chromatograpy with 5 mL of chloroform-methanol (7: 3) as eluent, instead of aluminum oxide column (100-200 mesh, 5 g, 1 cm) chromatograpy eluted successively with chloroform and the chloroform-methanol (7:3) (20 mL each), was applied to enrich matrine and oxymatrine in Sophora flavescens. Also, the optimization of the HPLC determination conditions with acetonitrile-ethanol absolute-3% phosphoric acid solution (84: 6: 10) as mobile phase, instead of acetonitrile-ethanol absolute -3% Phosphoric acid solution (80: 10: 10) recorded in Chinese Pharmacopoeia 2010 Edition, was more suitable for determination of matrine and oxymatrine in S. flavescens. This method has advantage of reducing sample handling time and solvent volume and increasing the accuracy and feasibility, which can simplify the procedure for determination of matrine and oxymatrine in S. flavescens.
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Quinolizinas / Medicamentos de Ervas Chinesas / Química / Cromatografia Líquida de Alta Pressão / Sophora / Alcaloides / Extração em Fase Sólida / Métodos Idioma: Zh Revista: China Journal of Chinese Materia Medica Ano de publicação: 2013 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Quinolizinas / Medicamentos de Ervas Chinesas / Química / Cromatografia Líquida de Alta Pressão / Sophora / Alcaloides / Extração em Fase Sólida / Métodos Idioma: Zh Revista: China Journal of Chinese Materia Medica Ano de publicação: 2013 Tipo de documento: Article