Experimental study of saponins from Tribulus terrestris on renal carcinoma cell line / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1271-1274, 2005.
Article
em Zh
| WPRIM
| ID: wpr-239703
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of saponins from Tribulus terrestris (STT) on the renal carcinoma cell (786-0) in vitro, and inhibitory mechanisms.</p><p><b>METHOD</b>Effects of SIT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-0 were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry (FCM).</p><p><b>RESULT</b>After the 786-0 was treated by STY, it was shown that: 1) A significant cytotoxic effect was observed by MTT assay; 2) Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3) The distribution of 786-0 on S phase was increased; 4.) The expression of Bcl-2 protein and cyclin D1 was decreased.</p><p><b>CONCLUSION</b>STT can significantly inhibit the growth of 786-0 in vitro, partially, by apoptosis.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Plantas Medicinais
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Saponinas
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Medicamentos de Ervas Chinesas
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Carcinoma de Células Renais
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Química
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Fase S
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Apoptose
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Proteínas Proto-Oncogênicas c-bcl-2
Limite:
Humans
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Article