Expression of early growth response gene-1 in macrophages stimulated by silicon dioxide / 中华病理学杂志
Chinese Journal of Pathology
; (12): 558-562, 2003.
Article
em Zh
| WPRIM
| ID: wpr-242139
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and localization of early growth response gene-1 (Egr-1) in macrophages after stimulation by silicon dioxide in vivo and in vitro and to discuss the role of Egr-1 in the development of silicosis.</p><p><b>METHODS</b>The expression of Egr-1 in animal model of silicosis was analyzed by using immunohistochemistry. Western-blot, immunofluorescence and RT-PCR analysis were used to detect the expression and localization of Egr-1 protein and the dynamic changes of Egr-1 mRNA in cultured macrophages RAW264.7, after stimulation by silicon dioxide.</p><p><b>RESULTS</b>In animal model with induced silicosis, there was an increased expression of Egr-1 in pulmonary macrophages. The expression levels peaked at the 14th day. In vitro, the transcription of Egr-1 increased in RAW264.7 macrophages during 15 to 240 minutes after the administration of silicon dioxide. The response peaked at 15 minutes and diminished to a minimal level at 480 minutes. Nuclear translocation was most apparent at 60 minutes, lasted till 120 minutes and diminished gradually. During the period from 60 to 120 minutes, the expression of Egr-1 protein also reached a peak.</p><p><b>CONCLUSIONS</b>Silicon dioxide can activate the nuclear transcription factor Egr-1 in vivo and in vitro in macrophages. Egr-1 may thus play an important pathogenetic role in the development of silicosis.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Fatores de Transcrição
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RNA Mensageiro
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Imuno-Histoquímica
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Linhagem Celular
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Regulação da Expressão Gênica
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Western Blotting
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Macrófagos Alveolares
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Ratos Wistar
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2003
Tipo de documento:
Article