Gene detection of severe acute respiratory syndrome-related coronavirus / 中华病理学杂志
Chinese Journal of Pathology
; (12): 212-214, 2003.
Article
em Zh
| WPRIM
| ID: wpr-242198
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a newly real-time RT-polymerase chain reaction assay for severe acute respiratory syndrome (SARS) related coronavirus in human whole blood.</p><p><b>METHODS</b>A pair of primers and a probe (molecular beacon) had been designed that were specific for the recognition of a highly conservative region between 15 301 and 15 480 of the SARS-related coronavirus polymerase gene sequences obtained from GenBank (G130027616).</p><p><b>RESULTS</b>In the real-time RT-PCR assay, the extent of SARS related coronavirus amplification was measured in terms of the increase in fluorescence during the amplification process. The 145 bp fragment of PCR product was further confirmed by conventional PCR assay and proved by DNA sequencing to be identical to the target sequence to which the probe was hybridized.</p><p><b>CONCLUSION</b>This assay has a broad application for clinical diagnosis and surveillance investigation.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Dados de Sequência Molecular
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Sequência de Bases
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Síndrome Respiratória Aguda Grave
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Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
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Diagnóstico
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Genética
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2003
Tipo de documento:
Article