Laboratory study on a rare case of chronic myeloid leukemia with ins(22;9)t(9;13) and Ph-negative / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 355-358, 2010.
Article
em Zh
| WPRIM
| ID: wpr-243356
Biblioteca responsável:
WPRO
ABSTRACT
The study aimed to examine a rare case of Philadelphia (Ph)-negative chronic myeloid leukemia (CML) with t(9;13). Chromosome samples were prepared after culture of bone marrow cells for 24 hours, the karyotypes were analyzed by G banding technique. Chromosome painting analysis was performed by using whole chromosome paints for chromosomes 9 and 22. Fluorescence in situ hybridization (FISH) was done with dual color dual fusion LSI bcr/abl probe. Bcr/abl transcripts were detected by real time fluorescence quantitative polymerase chain reaction (RQ-PCR). As a result, G banding analysis showed a karyotype of 45, XX, der(9)t(9;13)(q34;q10), -13[20]. FISH assay using LSI bcr/abl DNA probe showed a red abl signal inserted into der(22) and a fusion signal of bcr/abl rearrangement was discovered. RQ-PCR detected high copies of bcr/abl transcripts. In conclusion, insertion of bcr/abl rearrangement was a rare variant t(9;22) and could be well detected by molecular techniques, however, regular cytogenetic banding technique and whole chromosome paintings may probably lead a misdiagnosis to such cases.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Cromossomos Humanos Par 9
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Cromossomos Humanos Par 13
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Cromossomos Humanos Par 22
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Leucemia Mielogênica Crônica BCR-ABL Positiva
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Coloração Cromossômica
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Leucemia Mieloide Crônica Atípica BCR-ABL Negativa
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Genética
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Cariotipagem
Limite:
Female
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Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2010
Tipo de documento:
Article