Effect of proteasome inhibitor MG-132 on L1210 cell apoptosis and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 321-325, 2010.
Article
em Zh
| WPRIM
| ID: wpr-243363
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the effect of proteasome inhibitor MG-132 on apoptosis of L1210 cells and its mechanism. L1210 cells were treated with MG-132 of different concentrations (0, 2.5, 5, 10, 10 micromol/L). Cell viability was tested by MTT assay, apoptosis rate was detected by using flow cytometry, activity of caspase 3 was detected by colorimetry, the expression of NF-kappaB nuclear protein was detected by Western blot. The results showed that the growth inhibition of L1210 cells treated for a same time (24 hours) was enhanced along with increasing of MG-132 concentrations (0, 2.5, 5, 10, 20 micromol/L); the inhibitory rate, apoptosis rate and activity of caspase 3 increased also along with raising of MG-132 concentrations; while the expression of NF-kappaB nuclear protein decreased along with raising of GM-132 concentrations. It is concluded that MG-132 can induce the apoptosis of L1210. The mechanism of apoptosis may be related to the down-regulation of the expression of NF-kappaB and the activation of caspase 3.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
NF-kappa B
/
Apoptose
/
Linhagem Celular Tumoral
/
Inibidores Enzimáticos
/
Caspase 3
/
Leupeptinas
/
Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2010
Tipo de documento:
Article