Effect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines / 대한암학회지
Journal of the Korean Cancer Association
; : 852-862, 2000.
Article
em Ko
| WPRIM
| ID: wpr-24523
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Previously, we reported that the expression of E-cadherin was significantly decreased according to the increase of the level of hepatocyte growth factor (HGF) in gastric cancer tissue. In this work, the effect of HGF on the cell-cell adhesion and intracellular distribution of E-cadherin in the gastric carcinoma cell lines were studied. MATERIALS ANDMETHODS:
Western blot analysis was performed to confirm the presence or abscence of c-Met and E-cadherin in SNU-1, 5, and 16 cells. Tyrosine phosphorylation of c-Met, E-cadherin, alpha-, beta-, gamma-catenins was checked by immunoprecipitation. The morphologic changes induced by HGF were studied with immunocytochemical staining. Functional proportion of E-cadherin was estimated by cell fractionation. The effect of HGF on cell proliferation and invasion was also assessed.RESULTS:
Among SNU-1, 5, and 16 cell lines, only SNU-16 cells expressed both E-cadherin and c-Met. A morphological change from epithelial shape to fibroblastic one was observed in the SNU-16 cells after treatment with HGF. In addition, E-cadherin expression of the SNU-16 cells was shifted from the membrane and to the cytoplasm, and the functional fraction of E-cadherin was decreased in the SNU-16 cells treated with HGF. On the other hand, HGF increased the proliferation and invasion of the SNU-16 cells.CONCLUSION:
These results suggest that HGF may regulate cell adhesion in gastric carcinomas via the cellular redistribution and functional change of E-cadherin.Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosforilação
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Neoplasias Gástricas
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Tirosina
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Caderinas
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Adesão Celular
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Fracionamento Celular
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Linhagem Celular
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Western Blotting
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Fator de Crescimento de Hepatócito
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Hepatócitos
Idioma:
Ko
Revista:
Journal of the Korean Cancer Association
Ano de publicação:
2000
Tipo de documento:
Article