Study on the mitochondrial DNA variation in patients with type 2 diabetes mellitus / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 167-172, 2007.
Article
em Zh
| WPRIM
| ID: wpr-247361
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between type 2 diabetes mellitus (T2DM) and the mutations in the fragment of mitochondrial DNA (mtDNA) from nucleotides 3153 to 3551, which have shown high frequency of point mutation.</p><p><b>METHODS</b>One hundred and ninety-one normal controls and 222 patients with T2DM were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), T-A cloning sequencing and denatured high performance liquid chromatography (DHPLC) techniques.</p><p><b>RESULTS</b>The prevalence of mtDNA mutations in the patient group (24.32%) was significantly higher than that in the control group (7.33%) (P < 0.05). Three novel mutations of A3209T, T3253G and A3467C were found, and C3497T was first reported in DM. Onset age, body mass index, fasting blood glucose, HbA1C, high density lipoprotein-cholesterol and diabetic nephropathy could be related to occurrence of mtDNA mutations (P < 0.05).</p><p><b>CONCLUSION</b>Mitochondrial DNA mutations might implicate T2DM in Wenzhou population, which should play an important role in the pathogenesis of T2DM.</p>
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Índice:
WPRIM
Assunto principal:
Polimorfismo de Fragmento de Restrição
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DNA Mitocondrial
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Análise Mutacional de DNA
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Reação em Cadeia da Polimerase
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Cromatografia Líquida de Alta Pressão
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Diabetes Mellitus Tipo 2
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Genética
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Mutação
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2007
Tipo de documento:
Article