Porphyromonas gingivalis induced interleukin-6 expression by Nod/Rip2-mediated signaling pathway / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 37-40, 2009.
Article
em Zh
| WPRIM
| ID: wpr-248315
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate into the signaling pathway of Porphyromonas gingivalis (P. gingivalis) on cytokine expression in human dental pulp cells (HDPC).</p><p><b>METHODS</b>Anaerobic method was employed to culture P. gingivalis, and then HDPC were intracellularly infected by P. gingivalis. The extraction of total RNA, real-time quantitative polymerase chain reaction (qPCR) and enzyme linked immunosorbent assay (ELISA) was used for mRNA expression of Nods and Rip2, protein secretion of interleukin-6 (IL-6).</p><p><b>RESULTS</b>HDPC expressed Nods, Rip2 mRNA and IL-6. The up-regulation of Nods and Rip2 mRNA started after P. gingivalis infection, reached maximal level at 2 h, and then decreased at 6 h; whereas elevated IL-6 was found when P. gingivalis infected.</p><p><b>CONCLUSION</b>P. gingivalis activate host innate immune responses in HDPC, and induce IL-6 production through Nod/Rip2-mediated signaling pathway.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
RNA Mensageiro
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Regulação para Cima
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Células Cultivadas
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Interleucina-8
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Interleucina-6
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Porphyromonas gingivalis
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Article