High levels of testosterone inhibit ovarian follicle development by repressing the FSH signaling pathway / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 723-729, 2015.
Article
em En
| WPRIM
| ID: wpr-250351
Biblioteca responsável:
WPRO
ABSTRACT
The effect of high concentrations of testosterone on ovarian follicle development was investigated. Primary follicles and granulosa cells were cultured in vitro in media supplemented with a testosterone concentration gradient. The combined effects of testosterone and follicle-stimulating hormone (FSH) on follicular growth and granulosa cell gonadotropin receptor mRNA expression were also investigated. Follicle growth in the presence of high testosterone concentrations was promoted at early stages (days 1-7), but inhibited at later stage (days 7-14) of in vitro culture. Interestingly, testosterone-induced follicle development arrest was rescued by treatment with high concentrations of FSH (400 mIU/mL). In addition, in cultured granulosa cells, high testosterone concentrations induced cell proliferation, and increased the mRNA expression level of FSH receptor (FSHR), and luteinized hormone/choriogonadotropin receptor. It was concluded that high concentrations of testosterone inhibited follicle development, most likely through regulation of the FSH signaling pathway, although independently from FSHR downregulation. These findings are an important step in further understanding the pathogenesis of polycystic ovary syndrome.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Testosterona
/
Receptores do FSH
/
Receptores da Gonadotropina
/
Receptores do LH
/
RNA Mensageiro
/
Transdução de Sinais
/
Regulação da Expressão Gênica no Desenvolvimento
/
Biologia Celular
/
Proliferação de Células
Limite:
Animals
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2015
Tipo de documento:
Article