Triton X-100 induces apoptosis in human hepatoma cell lines
Yonsei Medical Journal
; : 52-59, 1997.
Article
em En
| WPRIM
| ID: wpr-25046
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WPRO
ABSTRACT
The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.
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Texto completo:
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Assunto principal:
Células Tumorais Cultivadas
/
Apoptose
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Octoxinol
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Carcinoma Hepatocelular
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Detergentes
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Fragmentação do DNA
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Neoplasias Hepáticas
Limite:
Humans
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
1997
Tipo de documento:
Article