Astragali Radix and Hedysari Radix molecular identification of SSR primers screening and fingerprints code / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1819-1822, 2016.
Article
em Zh
| WPRIM
| ID: wpr-250483
Biblioteca responsável:
WPRO
ABSTRACT
Leguminous related SSR primers were collected, core primers used for Astragali Radix and Hedysari Radix identification were screened and validated by using molecular marker techniques. 6 core primers were selected from 101 pairs of primers, the molecular weight of PCR products was 100-500 bp, which formed 7-12 electrophoresis bands with 55 amplified loci. The percentage of polymorphic loci was 100%, and the average polymorphism information content was 0.371. According to the results of cluster analysis, obtained core primer could completely distinguish 62 mixture samples of Astragali Radix and Hedysari Radix in similarity coefficient of 0.46. Core primers and the corresponding characteristics from gel electrophoresis were tagged. The results provide identification basis for Astragali Radix and Hedysari Radix.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2016
Tipo de documento:
Article