Application of spectral karyotyping to cytogenetic analysis in acute myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology
; (12): 454-457, 2011.
Article
em Zh
| WPRIM
| ID: wpr-251536
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the value of spectral karyotyping (SKY) in cytogenetic analysis of acute myeloid leukemias (AML).</p><p><b>METHODS</b>Nine AML patients were analyzed by R-banding and SKY. MLL, PML-RARalpha, AML1-ETO fusion genes were detected by dual fusion- fluorescence in situ hybridization (D-FISH).</p><p><b>RESULTS</b>All 9 samples were successfully hybridized. SKY identified structural aberrations including 9q -, t(15;17) and ins(10;17) (q22;p11p12) ; and some numeral abnormalities. The results of SKY confirmed those of R-band karyotyping and D-FISH; with more accurate localization.</p><p><b>CONCLUSION</b>SKY appears to be fairly stable, accurate and sensitive, for AML cytogenetic study.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Leucemia Mieloide Aguda
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Análise Citogenética
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Cariotipagem Espectral
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Genética
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Cariotipagem
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2011
Tipo de documento:
Article