Expression of human ermap gene in umbilical cord blood mononuclear cells during differentiation and development towards erythroid lineage / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 328-332, 2008.
Article
em Zh
| WPRIM
| ID: wpr-253325
Biblioteca responsável:
WPRO
ABSTRACT
The aim of study was to explore the potential of human erythroid membrane associated protein (ERMAP) gene in erythroid cell differentiation and development, mononuclear cells (MNCs) were isolated from umbilical cord blood and induced to erythroid cell differentiation by SCF, IL-3 and EPO. The cell morphology was observed by using optical microscopy, the positive rate of cells was counted by biphenylamine staining and the ratios of CD36+/CD235a-, CD36+/CD235a+, CD36-/CD235a+ cells were detected by flow cytometry, the change of human ermap gene expression level was analyzed by using fluorescent quantitative PCR (FQ-PCR). The results showed that the ermap gene expression level increased while MNCs were induced to erythroid lineage after treatment with SCF, IL-3 and EPO. It is concluded that the human ermap gene plays an important role in differentiation and development of erythroid cells.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Antígenos de Grupos Sanguíneos
/
Leucócitos Mononucleares
/
Diferenciação Celular
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Células Cultivadas
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Reação em Cadeia da Polimerase
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Biologia Celular
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Células Eritroides
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Sangue Fetal
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Butirofilinas
/
Genética
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2008
Tipo de documento:
Article