Adsorption condition optimization for anti-HBsAg Fab fragment separation and purification from E. coli using Streamline SP / 南方医科大学学报
Journal of Southern Medical University
; (12): 409-413, 2006.
Article
em Zh
| WPRIM
| ID: wpr-255298
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To optimize the adsorption condition of cation-exchange chromatographic media Streamline SP for separation and purification of anti-HBsAg Fab fragment from E. coli.</p><p><b>METHODS</b>The adsorption of the target protein for separation and purification by the cation-exchange chromatographic media Streamline SP was tested using test tube method in balanced buffer solution with different pH values and ion concentrations. The adsorption effect was then verified by cation-exchange chromatography using 1-ml Streamline SP prepacked column and 28-ml Streamline SP self-assembly column.</p><p><b>RESULTS</b>According to the experiment results of test tube method, the loading buffer with pH of 4.4 and ionic concentration of 100 to 600 mmol/L could achieve optimal target protein adsorption effect by cation-exchange chromatographic media Streamline SP, as verified by cation-exchange chromatography with 1-ml SP prepacked column and 28-ml Streamline SP self-assembly column.</p><p><b>CONCLUSION</b>The optimal condition of cation-exchange chromatography selected by test tube method can be applied for separation and purification of anti-HBsAg Fab fragment from E. coli.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Fragmentos Fab das Imunoglobulinas
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Resinas de Troca de Cátion
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Cromatografia por Troca Iônica
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Adsorção
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Alergia e Imunologia
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Escherichia coli
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Genética
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Anticorpos Anti-Hepatite B
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Antígenos de Superfície da Hepatite B
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Article