Regulative effect of IFN-gamma on the Fas/Fas L system of cholangiocarcinoma cells / 中华预防医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To detect the regulative effects of IFN-gamma on the expression of Fas and FasL of cholangiocarcinoma cells.</p><p><b>METHODS</b>We studied that the expression of Fas and FasL gene by the human cholangiocarcinoma cell line QBC939 by RT-PCR, Western blot, immunohistochemistry. At the same time, we investigated the regulative effect of IFN-gamma on them.</p><p><b>RESULTS</b>Fas and FasL mRNA and protein were expressed by cholangiocarcinoma cells. We also found IFN-gamma could upregulate the expression of the two genes (P < 0.01). However, IFN-gamma could also downregulate the ability of them to make Jurkat cells apoptotic. With the increasing of dosage and time, the effect was enhanced.</p><p><b>CONCLUSIONS</b>IFN-gamma could regulate the expression of Fas and FasL by cholangiocarcinoma cells, therefore it could reduce the ability of cholangiocarcinoma to occur immune escape. This provides new theoretical basis for immunological therapy of cholangiocarcinoma.</p>