The role of myosin light chain kinase in intestinal epithelial barrier dysfunction due to hypoxia / 中华烧伤杂志
Chinese Journal of Burns
; (6): 57-60, 2009.
Article
em Zh
| WPRIM
| ID: wpr-257443
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of myosin light chain kinase (MLCK) in intestinal epithelial barrier dysfunction after hypoxia.</p><p><b>METHODS</b>The Caco-2 monolayers developed with Transwell inserts were exposed to hypoxia for 0 h (NC group), 2, 6, 8, 12 and 24 h (H group), and 6 h hypoxic specimens were treated with 100 mol/L ML-9 (T group). The transepithelial electrical resistance (TER) of monolayers was measured with an ohmmeter. The tight junction protein ZO-1 of monolayers was analyzed by immunofluorescence assay. The protein expressions of phosphorylated myosin light chain (p-MLC) and MLCK were detected by Western blotting.</p><p><b>RESULTS</b>The TER of monolayers in H group at 6, 8, 12 and 24 h was 422 +/- 17, 427 +/- 27, 403 +/- 40 and 426 +/- 22 ohms respectively, which was significantly lower than that of NC group (451 +/- 27 ohms, P < 0.05). The TER of monolayers in T group was 558 +/- 110 ohms, which was significantly higher than that in H group at each time point ( P < 0.01). The ZO-1 of monolayers in H group at 6 h was irregular in arrangement, with interruptions and rugae, and sawtooth. These abnormalities were ameliorated in T group (regular in arrangement, with little or without ruga and sawtooth). The protein expressions of p-MLC and MLCK in H group at each time point were higher than those in NC group.</p><p><b>CONCLUSIONS</b>Intestinal epithelial barrier dysfunction after hypoxia can be mediated by MLCK.</p>
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Índice:
WPRIM
Assunto principal:
Quinase de Cadeia Leve de Miosina
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Cadeias Leves de Miosina
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Células CACO-2
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Biologia Celular
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Epitélio
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Absorção Intestinal
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Mucosa Intestinal
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Intestinos
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Hipóxia
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Burns
Ano de publicação:
2009
Tipo de documento:
Article