Isolation and identification of berberine from endophytic fungi HL-Y-3 / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2998-3001, 2016.
Article
em Zh
| WPRIM
| ID: wpr-258429
Biblioteca responsável:
WPRO
ABSTRACT
The endophytic fungi HL-Y-3, which was isolated from the healthy leaves of Coptis chinensis, produced berberine when grown in the PDA culture medium. The presence of berberine was confirmed by the chromatographic and spectroscopic analyses. The yield of berberine was recorded as 9.313 μg•g⁻¹ by HPLC. The strain HL-Y-3 was identified as Alternaria sp.by morphological observation and 5.8S rDNA-ITS sequence analysis.The separation and purification of constituents were performed by PTLC. The mass spectrometry (MS) of the analyte was shown to be identical with authentic berberine.Further analysis with nuclear magnetic resonance (NMR) spectroscopy to showed that the chemical structure of the fungal berberine was identical with authentic berberine. The research provided new resources for the utilization of berberine.
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Índice:
WPRIM
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2016
Tipo de documento:
Article