Enhancement of Virus Replication in An Influenza A Virus NS1-Expresssing 293 Cell Line / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 224-228, 2016.
Article
em En
| WPRIM
| ID: wpr-258830
Biblioteca responsável:
WPRO
ABSTRACT
The nonstructural protein 1 (NS1) of influenza A virus, which is absent from the viral particle, but highly expressed in infected cells, strongly antagonizes the interferon (IFN)-mediated antiviral response. We engineered an NS1-expressing 293 (293-NS1) cell line with no response to IFN stimulation. Compared with the parental 293 cells, the IFN-nonresponsive 293-NS1 cells improved the growth capacity of various viruses, but the introduction of NS1 barely enhanced the propagation of Tahyna virus, a negative-strand RNA virus. In particular, fastidious enteric adenovirus that replicates poorly in 293 cells may grow more efficiently in 293-NS1 cells; thus, IFN-nonresponsive 293-NS1 cells might be of great value in diagnostic laboratories for the cultivation and isolation of human enteric adenoviruses.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Vírus da Influenza A
/
Fisiologia
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Cultura de Vírus
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Replicação Viral
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Linhagem Celular
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Regulação da Expressão Gênica
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Proteínas não Estruturais Virais
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Células HEK293
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Genética
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Metabolismo
Limite:
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2016
Tipo de documento:
Article