Establishment of a bortezomib-resistant myeloma cell line and differential proteins analysis by MALDI-OF-MS / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 445-452, 2009.
Article
em Zh
| WPRIM
| ID: wpr-259284
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a bortezomib-resistant myeloma cell line and to investigate its mechanism.</p><p><b>METHODS</b>Bortezomib-resistant NCI-H929 cell line (NCI-H929B) was obtained by stepwise increasing extracellular concentrations of bortezomib over a period of 8 months. The biological characteristics of NCI-H929 and NCI-H929B were observed. Proteins from NCI-H929B cell and NCI-H929 cell were extracted, run on two-dimensional gel electrophoresis. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and mass spectrometry (MS) were used to identify proteins. Western blot was used to further verify differential proteins.</p><p><b>RESULT</b>Bortezomib-resistant cell line NCI-H929B was established. NCI-H929B exhibits a 23.5-fold level of resistance to bortezomib as compared to the parental cell line NCI-H929. There were no significant differences in cellular biology of cell growth curve and cell cycle distribution between NCI-H929 and NCI-H929B cell lines.Whole proteins of NCI-H929 and NCI-H929B myeloma cell lines were extracted by two-dimensional gel electrophoresis. Gel-image analysis revealed that there were 17 differential protein spots. A total of 14 differential protein spots were successfully identified by MALDI-TOF-MS. The result of Western blot was consistent with 2-DE.</p><p><b>CONCLUSION</b>A bortezomib-resistant human myeloma cell line NCI-H929B was successfully established. The differentially expression of proteomes may be useful for study of the bortezomib-resistant mechanisms and the molecular markers of MM.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
Pirazinas
/
Ácidos Borônicos
/
Proteínas do Mieloma
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Resistencia a Medicamentos Antineoplásicos
/
Proteoma
/
Linhagem Celular Tumoral
/
Bortezomib
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Article