Production of interleukin-1beta, interleukin-6 and tumor necrosis factor a in cultured human fibroblast with stimulation of abstract from Actinomyces naeslundii ATCC19246 / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the production of interleukin (IL)-1beta, IL-6 and tumor necrosis factor alpha (TNFalpha) from stimulated human fibroblast with abstract from cell wall of Actinomyces naeslundii ATCC19246.</p><p><b>METHODS</b>The abstract from the cell wall from Actinomyces naeslundii were extracted and purified with the method of purifying lipoteichoic acid(LTA) and stimulated the THP-1 with three different concentrations (1, 10, 100 mg/mL). The level of IL-1beta, IL-6 and TNFalpha in the supernatant was quantitatively analyzed by ELISA. Results Abstracts at the concentrations of 10, 100 mg/mL significantly produced IL-1beta, IL-6 and TNFalpha, especially 10 mg/mL.</p><p><b>CONCLUSION</b>The abstract from cell wall of Actinomyces naeslundii may significantly increase IL-1beta, IL-6 and TNFalpha level in the supernatant of THP-1, and the increasing level is different with the concentrations.</p>