Knockdown of STAT3 expression using siRNA inhibits the growth of prostate cancer cell lines / 中华男科学杂志
National Journal of Andrology
; (12): 29-37, 2005.
Article
em Zh
| WPRIM
| ID: wpr-267763
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of pSilencer 1.0-U6-siRNA-STAT3 on the growth of PC3 and LNCaP cells.</p><p><b>METHODS</b>Three pairs of DNA template coding siRNA were synthesized against STAT3 to reconstruct pSilencer 1.0-U6-STAT3-siRNA, which was transfected into PC3 and LNCaP cells. The STAT3 expression in PC3 cells and LNCaP were transfected with pSilencer 1.0-U6-siRNA-STAT3, and it was detected by Western blot and Northern blot. MTT and FCM were used to observe the growth-inhibiting ratio and apoptosis in PC3 cells.</p><p><b>RESULTS</b>Western blot and Northern blot analyses demonstrated that pSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit the expression of STAT3 in PC3 and LNCaP cells; MIT and FCM results showed that it could suppress the growth of PC3 cells and LNCaP and induce apoptosis of PC3 cells in vitro.</p><p><b>CONCLUSION</b>PSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of PC3 and LNCaP cells and induce the apoptosis of PC3 cells.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Plasmídeos
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Neoplasias da Próstata
/
Transcrição Gênica
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RNA Mensageiro
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Apoptose
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RNA Interferente Pequeno
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Linhagem Celular Tumoral
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Fator de Transcrição STAT3
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Genética
Limite:
Humans
/
Male
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2005
Tipo de documento:
Article