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Construction of eukaryotic expression vector for VEGF165 gene and its expression in rat bladder smooth muscle cells / 南方医科大学学报
Article em Zh | WPRIM | ID: wpr-268055
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct an eukaryotic expression vector for vascular endothelial growth factor (VEGF) 165 gene and obtain VEGF expression in rat bladder smooth muscle cells.</p><p><b>METHODS</b>VEGF165 cDNA was cloned into the eukaryotic expression vector pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/VEGF165 was transfected into the rat bladder smooth muscle cells by electroporation. VEGF expression in the cells was determined by RT-PCR and immunofluoresence assay, and the biological activity of VEGF in the supernant of the transinfected cell culture was tested by MTT assay.</p><p><b>RESULTS AND CONCLUSION</b>VEGF expression was obtained in the transinfected cells, and the supernant of the transinfected cell cultures stimulated the proliferation of the endothelial cells.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Farmacologia / Bexiga Urinária / Transfecção / Expressão Gênica / Linhagem Celular / Células Cultivadas / Imunofluorescência / Clonagem Molecular / Meios de Cultivo Condicionados / DNA Complementar Limite: Animals / Humans Idioma: Zh Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Farmacologia / Bexiga Urinária / Transfecção / Expressão Gênica / Linhagem Celular / Células Cultivadas / Imunofluorescência / Clonagem Molecular / Meios de Cultivo Condicionados / DNA Complementar Limite: Animals / Humans Idioma: Zh Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Article